您好！歡迎訪問上海起發(fā)實驗試劑有限公司網(wǎng)站！
全國服務咨詢熱線:
15921799099
15921799099
| 型號 | 載體名稱 | 出品公司 | 載體用途 |
| VMA0373 | pSicoR pGK puro | Addgene | 慢病毒載體 |
Backbone size （bp）: 7700
Modifications to Backbone: Puromycin is expressed from this plasmid as a selectable marker. Puromycin and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Cloning site 5': HpaI
Cloning site 3': XhoI
5' sequencing primer: mU6-F
pSicoR PGK Puro allows for conditional （Cre-Lox）, stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression. See pSicoR and pSico PGK Puro for more information.
The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map for pSico and on the Jacks Lab
地址：上海浦東川沙鎮(zhèn)川沙路6619號上海起發(fā)實驗試劑有限公司
郵箱：xs1@78bio.com
傳真：021-50724961
電話
QQ咨詢
4006551678
當前位置：